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Purified anti-PERK Phospho (Ser713) [Poly6494]; Isotype: Rabbit Polyclonal IgG; Reactivity: Human; Apps: WB; Size: 100 μl
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Image Search Results
Journal: International journal of molecular medicine
Article Title: FGF21 attenuates hypoxia‑induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress.
doi: 10.3892/ijmm.2018.3705
Figure Lengend Snippet: Figure 4. FGF21 alleviates the hypoxia‑induced ERS by modulating the expression of ERS‑related proteins in HPAECs. HPAECs were treated as indicated and protein expression levels were examined by western blotting. Representative blots and quantification are shown for (A) BiP, (B) p‑PERK and PERK, (C) the ratio of p‑PERK/PERK, (D) CHOP, (E) Bcl‑2. (F) Relative expression of the ERS‑dependent apoptotic protein caspase‑4 in HPAECs was analysed by western blotting. GAPDH was used as an internal control. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (n=3). **P<0.01 vs. N group; ##P<0.01 vs. the H group; &&P<0.01 vs. the H+T group. FGF21, fibroblast growth factor 21; ERS, endoplasmic reticulum stress; HPAECs, human pulmonary arterial endothelial cells; BiP, binding immunoglobulin protein; p‑, phosphorylated; PERK, protein kinase R‑like endoplasmic reticulum kinase; CHOP, transcrip- tion factor C/EBP homologous protein; Bcl‑2, B cell lymphoma-2; N, normoxia; H, hypoxia; F, FGF21; S, salubrinal; T, tunicamycin.
Article Snippet: Rabbit antibodies against
Techniques: Expressing, Western Blot, Control, Standard Deviation, Binding Assay
Journal: International journal of molecular medicine
Article Title: FGF21 attenuates hypoxia‑induced dysfunction and apoptosis in HPAECs through alleviating endoplasmic reticulum stress.
doi: 10.3892/ijmm.2018.3705
Figure Lengend Snippet: Figure 6. Effect of FGF21 on NO and ET‑1 secretion in HPAECs. (A) HPAECs were treated as indicated and at the end of treatment, the cell culture medium was collected and assayed by ELISA for the levels of secreted NO and (B) the levels of secreted ET‑1. Data are expressed as the mean ± standard deviation (n=3). **P<0.01 vs. the N group; #P<0.05 and ##P<0.01 vs. the H group; &&P<0.01 vs. the H+T group. (C) Schematic of the proposed mechanism by which FGF21 attenuates hypoxia‑induced apoptosis and dysfunction by alleviating ERS in HPAECs. FGF21, fibroblast growth factor 21; NO, nitric oxide; ET‑1, endothelin‑1; HPAECs, human pulmonary arterial endothelial cells; ERS, endoplasmic reticulum stress; N, normoxia; H, hypoxia; F, FGF21; S, salubrinal; T, tunicamycin; BiP, binding immunoglobulin protein; p‑, phosphorylated; PERK, protein kinase R‑like endoplasmic reticulum kinase; CHOP, transcription factor C/EBP homologous protein; Bcl‑2, B cell lymphoma-2.
Article Snippet: Rabbit antibodies against
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation, Binding Assay